何妨吟嘯且徐行 Dedicate to life

(p/s: this is my first entry of english log, definitely i have a very poor command of english language. I even forget how i passed the essay writing tests during secondary school). However, i would like to try to write some short paragraphs here for the sakes of getting more people visit to this blog. In addition, possibly this is a good platform for me to practice my english, practices make perfect. Anyway, most of writings will orientate in the radiancy of my academic study)

Today is 13 June 2005. When i check the last year calendar just now, i found that i registered officially as a master student at 21 June 2005. Time and tide wait for no man.

These few months i work at a totally new environment, after time and time, i am getting adapted in this new environment, laboratory of microbiology. After working for 2 months, it is the time for me to reorganize my experimental works in order to get a clearer mind for further works.

Laboratory works had been done
(Some works will be repeated from time to time depend on the necessities)

I Preparation of materials and apparatus

1. Sampling and storage of wastewater (oxidation ponds)
2. Washing and sterilization of apparatus and medium
3. Preparation of medium

a. Culture medium — i. nutrient broth ii. nutrient agar iii. filter-sterilized wastewater b. Selective medium — i. nitrate selective agar ii. phosphate selective agar iii. sulphate selective agar iv. medium for ammonia oxidizers

II Investigation of water quality of the wastewater
Sampling location: oxidation pond KTC, oxidation pond Desa Bakti
Sampling point: 8
Parameter determined: pH, turbidity, TSS, BOD, COD, total nitrogen, ammonia, nitrate, phosphate, sulphate, ferrous iron

III Isolation of bacteria from wastewater
Medium: nutrient agar
Technique: 1. serial dilution inoculation

2. streak plate

IV Screening of bacteria for nutrients removal
i. Screening of isolated pure culture in selective medium (nitrate, phosphate,

sulphate)

V Preparation of bioblock
Constituents: cement (ordinary Portland cement), small aggregates (laterite and

granite), sand, zeolite (natural zeolite, mordenite and synthetic zeolite, zeolite Y)
Size of aggregates: 2.8 to 5.6 mm
Size of sand: 150 μm to 2.8 mm
Procedures: 1. mould preparation

2. mixing of composite components 3. curing of bioblock

Bioblock has been prepared:
No. Code Aggregates Aggr. (kg) Sand (kg) Cement (kg) Water (kg)
1. BB-G1 Granite 0.855 0.285 0.120 0.084
2. BB-L1 Laterite 0.855 0.285 0.120 0.084
3. BB-L2 Laterite 0.735 0.285 0.240 0.168
4. BB-L3 Laterite 0.795 0.285 0.180 0.126
5. BB-L4 Laterite 0.825 0.285 0.150 0.105
Water to cement ratio = 0.70
Total weight of mixing components = 1.260 kg